These are fixed, permeabilized, and fluorescently-stained muntjac skin fibroblasts in either interphase or a phase of mitosis. The phases, going from top left to bottom right, are: interphase, prophase, metaphase, anaphase, and telophase.
During interphase, a eukaryotic cell multiplies its inventory of organelles and replicates its DNA (pink), growing in size.1 Next, mitosis is activated and the cell enters prophase, during which its replicated DNA is supercoiled into chromosomes comprised of identical, paired sister chromatids. Eventually, the cell’s nuclear envelope disintegrates, and special microtubules (green) linking the kinetochores of chromatids to the cell’s poles develop. The cell then enters metaphase, during which the centromeres of its chromosomes become aligned in a plane at its equator. After passing a key mitotic checkpoint, the cell enters anaphase, during which the paired sister chromatids separate and are pulled to opposite poles. Finally, in telophase, nuclear envelopes and nucleoli re-form around each pole’s collection of DNA, chromatids uncoil back into chromatin, and the cell divides in two. Each daughter cell is at the start of interphase, and the cell cycle repeats.2
Alexa Fluor® 350 phalloidin labeled actin filaments (blue) throughout each cell. An anti-α-tubulin antibody prelabeled with the Zenon® Alexa Fluor® 488 Mouse IgG1 Labeling Kit marked microtubules (green), revealing their role in mitosis. The anti–Cdc6 peptide antibody prelabeled with the Zenon® Alexa Fluor® 647 Mouse IgG1 Labeling Kit stained DNA (pink) in both chromatin and chromosomes.
On the Road with Grizzly Bear’s Ed Droste
For more photos from Ed and Grizzly Bear, follow him at @edroste and be sure to check out Ed’s Photos of You section on your mobile device.
In addition to being an avid Instagrammer, Ed Droste (@edroste) also happens to be a songwriter and singer in Grizzly Bear, an indie-rock band from Brooklyn, New York. The band has been playing shows across the United States after the debut of their latest album, and Ed has been sharing his behind-the-scenes perspective of life on the road as they go.
Midway through their tour we launched Photos of You, providing Ed with an incredible new view of Grizzly Bear’s life on the road—the fans’ perspective: “I’ve always used Instagram as a public photo album of my travels, and the Photo Map is one of my favorite elements in terms of remembering exactly where I was when I took certain pictures years ago, but with Photos of You I get a whole new perspective on tours and my journeys, which is really cool to see.”
Ed’s Photos of You section has not only given him the opportunity to view photos of the band’s performance from the audience’s perspective, but it’s also allowed him to re-connect with Grizzly Bear fans. “I love meeting people as much as I can after shows. Naturally, I can’t document and remember every person I meet, but this has definitely jogged my memory on meeting some really lovely and cool fans and conversations.”
What have you discovered through Photos of You? Starting tomorrow, the Photos of You section will be visible to everyone who can currently see your profile. Read more about Photos of You here.